Product overview

Just add DNA to express analysis-ready antibodies or target proteins in hours!

In cell-free protein expression systems, gene transcription (TX) and translation (TL) are unbound from constraints required for cell culture-based processes. myTXTL systems offer a versatile and easy-to-use cell-free expression platform for protein synthesis, enzyme engineering, and synthetic biology processes in industry and academia.

The cell-free myTXTL systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures, like bacteriophages, under open-reaction conditions. This enables easy manipulation, addition of chaperone molecules and straightforward downstream processing of synthesized proteins, enzymes and phages.

• Accelerate your workflow with cell-free protein expression tools that let you screen more variants in less time. Accomplish in two days what would take 3 weeks with in-vivo methods.
• Scale up production with high-throughput solutions that enable increased yield with reliable performance.
• Achieve superior results with tools designed for ease of use, backed by scientific experts available to help optimize your protocols and ensure your success.
• Perform protein expression not possible with in-vivo methods, such as synthesizing toxic proteins that prevent cell survival.

Technology

myTXTL utilizes the endogenous transcription (TX) and translation (TL) machinery from E. coli. Protein expression is initiated by simply adding DNA to the master mix and target protein activity can be measured in as little as 15 minutes.

Based on a system originally developed by Prof. Vincent Noireaux at the University of Minnesota, our revolutionary myTXTL technology has been further refined for greater robustness and manufacturability to deliver reliable, consistent performance.

Two systems, choose what’s right for you:

• myTXTL Pro Kit: High yield system for proteins that do not require disulfide bond formation
• myTXTL Antibody/DS Kit: Engineered for the expression of antibodies and disulfide-bond containing proteins, can also express proteins without disulfide bonds

Advantages

• Use linear or plasmid DNA templates – skip cloning!
• Supports any promoter system used for protein expression in E. coli, including T7, no need for recloning into a specialized plasmid.
• High throughput applications, 10s or 1000s of reactions.
• Open reaction allows dilution into activity assays, no purification.
• Adjustable reaction volumes to suit your needs.

myTXTL Pro Kit

myTXTL cell-free expression kits enable rapid protein expression, with many advantages over in vivo protein expression including the ability to deliver assayable protein in a single day and to skip time-consuming cloning and lysis/purification steps.

The myTXTL Pro Kit provides maximal expression of proteins without disulfide bonds, by leveraging the combined advantages of previous myTXTL kits.

Each myTXTL Pro Kit contains the Pro Master Mix, the Helper Plasmid (for T7 RNA polymerase expression), and the T7 deGFP Control Plasmid (for T7-promoter based expression of deGFP). To express proteins that contain disulfide bonds, please see our myTXTL Antibody/DS Kit.

• High yield – Produces enough protein for activity, screening, and biophysical assays (e.g. >1 mg/mL).
• Single day workflow– Enables quick turnaround times with an automation friendly procedure starting from linear or plasmid templates.
• Reduce processing – Skip lysis and purification to simplify protein expression, saving time and effort in downstream processing.
• No cloning required – The use of linear DNA eliminates the need for cloning, streamlining the expression process and saving 1-2 weeks versus in vivo expression.
• Express toxic proteins – The system supports the expression of toxic proteins, allowing researchers to study and characterize a wide range of target molecules with one kit.
• Maximum versatility – Supports constitutive and inducible E.coli and phage (T7) promoters, no unique plasmid system required.

myTXTL Antibody/DS Kit

The myTXTL Antibody/DS Kit from Daicel Arbor Biosciences is a cutting-edge solution for efficient, high-yield expression of disulfide bond-containing proteins (such as antibodies) as well as non-disulfide bond-containing proteins. This kit provides industry-leading yields of disulfide bond proteins, even for complex constructs such as Fab and IgG. All without sacrificing the speed and convenience that researchers have come to appreciate from the myTXTL system—simply drop in your linear or plasmid DNA and go!

Each myTXTL Antibody/DS Kit includes the DS Master Mix, the Helper Plasmid (for T7 RNA polymerase expression), and the T7 GLuc Control Plasmid (for T7 promoter-based expression of GLucDura).

• High yield – Rapidly produces complex proteins with and without disulfide bonds, in amounts sufficient for functional studies such as ELISA or SPR.
• Single day workflow – Enables quick turnaround times with an automation-friendly procedure starting from linear or plasmid templates.
• Reduce processing – Skip lysis and purification to simplify protein expression, saving time and effort in downstream processing.
• No cloning required – The use of linear DNA eliminates the need for cloning, streamlining the expression process and saving 1-2 weeks versus in vivo cycles.
• Maximum versatility – Supports constitutive and inducible E.coli and phage (T7) promoters, as found in common E.coli protein expression plasmids.