WATCHMAKER DNA LIBRARY PREP KITS
- Up to a 90% reduction in sequence artifacts improves assay accuracy – critical for highly sensitive applications
- Improved library amplification polymerase error rates and even UMI family coverage enable rare mutation detection
- Robust fragmentation and library prep efficiency support the use of clinically relevant sample types, such as low input and degraded
- Highly tunable fragmentation delivers consistent library sizes over a wide input range (< 1 ng to 500 ng) and between library batches
- Streamlined workflow delivers PCR-free libraries in under 90 minutes and scales easily to high sample numbers and automation platforms
- Uniform sequence coverage improves sequencing efficiency
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Accuracy-at-scale
Watchmaker DNA Library Prep Kits with Fragmentation enable highly sensitive clinical and translational applications to access meaningful insights from a broad range of biological sample types, including ultra-low inputs and FFPE, with an unparalleled combination of accuracy and scalability.
The workflow harnesses the process benefits of enzymatic fragmentation, such as ease of automation, increased scalability, and preservation of low input samples, while mitigating the formation of associated library preparation artifacts, including false chimeric reads and hairpin artifacts, that can convolute variant calling. Further, use of the Equinox polymerase delivers ultra-high-fidelity, low-bias library amplification.
Applications
- Somatic mutation calling and other low-frequency variant detection NGS assays, including those utilizing challenging samples such as FFPE
- Inherited disease sequencing
- Human whole genome sequencing (WGS), including PCR-free
- Whole exome sequencing (WES)
- Single cell analysis
- Metagenomic analysis
- Bulk RNA sequencing (using cDNA as input)
- Viral genome sequencing
- Microbial WGS
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Technical specifications
| Specification | Value | ||
|---|---|---|---|
| Kit contents | Frag/AT Buffer Frag/AT Enzyme Mix Ligation Master Mix Equinox Library Amplification Master Mix (2X, optional) P5/P7 Primer Mix (10X, optional) |
||
| Shipping conditions | Ice packs | ||
| Storage | -20°C ±5°C | ||
| Shelf life | 12 months | ||
Ordering information
| Description | 24 rxn | 96 rxn |
| Watchmaker DNA Library Prep Kit with Fragmentation
incl. Equinox Library Amplification Master Mix (2X) and P5/P7 Primer Mix (10X) |
7K0019-024 | 7K0019-096 |
| Watchmaker DNA Library Prep Kit with Fragmentation (PCR-free) |
7K0013-024 | 7K0013-096 |
| Watchmaker DNA Library Prep Kit with Fragmentation (w/o primers); incl. Equinox Library Amplification Master Mix (2X) |
7K0022-024 | 7K0022-096 |
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