FastGene qFYR Real-Time PCR System
Real-time fluorescent quantitative PCR system
- Fast detection − Probe & dye-based assays
- Multiplex qPCR − 4+1 channels
- 4 different colour channels
- Plus an additional second FAM/SYBR channel for fast dual mode melt curve analysis
- Standard plate format − 96-well block with gradient function
- Highest sensitivity
- Outstanding homogeneity across all wells of a 96-well plate
- Software included − Easy-to-use and intuitive
- Integrated Precision Melt Analysis Tool (for probe-based allelic discrimination)
- Many applications − Gene expression analysis, genotyping, HRM (high resolution melt), pathogen detection, genetic analysis
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Product overview
Highest precision instrument
The FastGene® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.
The device for multiple applications
The FastGene® qFYR was developed to meet highest laboratory standards and deliver reliable performances for various real-time qPCR applications:
- Gene expression analysis
- Absolute and relative quantification
- Endpoint qualitative analysis
- Genotyping
- Gene mutation analysis
- Pathogen detection
- GMO detection
- Protein stability screening
- miRNA analysis
- Melting curve analysis
Innovative Technology
High sensitivity optical design
The FastGene® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.
Superior thermal cycler
The FastGene® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.
Fast scanning mode − 4+1 channels
4 different fluorescence channels and a double FAM/SYBR channel enabling fast measurements.
Due to the small distance between the scanning head and the plate, the FastGene® qFYR has an outstanding level of sensitivity and virtually no cross talk between the individual wells.
Four different colour channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 sec).
The double FAM/SYBR channel allows to perform melt curve and high resolution melt experiments in fast dual mode, which halves the measuring time in this channel.
Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.
Specifications
Thermal cycler
| Block capacity | 96 |
| Sample volume | 1–50 µL |
| Heating and cooling method | Peltier (6 temperature control modules) |
| Temperature control technology | Hollow-out module combined with edge temperature compensation technology |
| The maximum heating and cooling rate of the block | 6.0 °C/s |
| The average heating and cooling rate of the sample | 4.0 °C/s |
| Temperature range | 4–100 °C |
| Temperature accuracy | ± 0.2 °C |
| Temperature uniformity | ± 0.2 °C |
| Temperature setting range | 4–100 °C |
| Heated lid | Electronic automatic lid |
| Gradient zones | 12 columns |
| Temperature gradient temperature range | 1–36 °C |
| Linear dynamic range | 10 orders of magnitude: 1-1010 copies |
| Software name | FastGene®qFYR Analysis Studio |
Optical detection
| Excitation light source | 4 single-color high-efficiency LEDs (maintenance-free, working life >100,000 hours) |
| Detector device | Highly sensitive PMT (photo multiplier tube) with Frensel lens |
| Scanning principle | Time-resolved real-time scan |
| Fluorescent channel number | 4 channels (2x FAM) |
| Application to fluorescent | Channel 1: FAM/SYBR Green etc. Channel 2: VIC/JOE/HEX/TET etc. Channel 3: ROX/Texas Red etc. Channel 4: Cy5 etc. Channel 5: FAM/SYBR Green etc. |
| Dye compatibility | FAM/SYBR Green VIC/HEX/TET/JOE ROX/Texas Red, Mustang Purple Cy5/LIZ |
| Detector position | Top of the block |
| Detection sensitivity | 1 copy of the target sequence |
| System sensitivity | Distinguishable 1.33-fold copy number difference in singleplex reactions |
| Detection time | Standard mode (full channel): 8.5 seconds/96-well plate; Fast mode (dual FAM): 4 seconds/96-well plate |
| Exitation/detection range | Excitation range: 455–650 nm; Scope of test: 510–715 nm |
Physical specifications
| Instrument specifications | Dimensions: 35 cm x 52 cm x 37 cm (length x wide x height) Weight: 25 kg |
| Environmental conditions | Temperature: 10–30 °C Humidity: ≤85 % RH no condensation Atmosheric pressure: 85.0 kPa ~ 106.0 kPa Altitude: < 2000 m |
| Power supply specification | Power supply voltage: AC 100–240 V Frequency: 50 Hz Power: 750 W |
| Data communication interface | USB ports |
Downloads
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