iconPCR
Individual well control. Faster prep. Better sequencing
World’s First Real-time Thermocycler with 96 individually controlled wells.
Gain control of your PCR at the individual well level with AutoNorm™. iconPCR’s AutoNorm enables customers to combine quantification and normalized pooling into one step to produce high quality libraries by ensuring individual reactions are amplified to the same levels.
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Product overview
The world’s first thermocycler with individually controlled wells and built-in AutoNorm™
iconPCR is the first and only thermocycler that intelligently adjusts amplification in real time—so you get normalized, sequencing-ready libraries in one automated step. Built-in AutoNorm technology eliminates the need for individual quantification and normalization, delivering the best possible data from every sample, regardless of input or quality.
NGS workflow improvement
Significantly streamline and improve NGS library preparation workflows
- Built-in AutoNorm eliminates pre and post-PCR quantification and normalization steps
- Works with your current kits
- Up to 50% less hands-on time
- 1 SPRI cleanup instead of 96 (for 96 samples)
Maximum flexibility for protocol development
- Linear Gradient: 96 different temperatures with >80°C range
- Each well is independently monitored at a millisecond frequency and actively controlled to a precision within 0.2°C of the required temperature.
Introduction to iconPCR
Specifications
iconPCR with AutoNorm™
| Thermocycling Format | 96-independently controlled wells |
| Sample Volume | 10-100 μl |
| Consumables Formats | 96 well plates and 8-tube strips |
| Optical System | Optical System |
| Sensitivity | Single molecule detection |
| Dynamic range | 10 orders of magnitude detection |
| Electrical Approvals | IEC61010-1, cTUVUS, CE, RCM |
| Dimensions (cm/in) | W 41/16, D 54/21.5, H 32/12.5 |
| Weight (kg) | 31 |
| Temperature Ramp Rate | 5℃/s |
| Heated Lid Temperature Range | 30-110℃ |
| Thermal accuracy | ±0.2℃ of target at 90℃ |
| Thermal Uniformity | 0.2℃ %CV over 96 wells |
| Gradient Range | 4-100℃ at 0.2℃ intervals |
| Programmable Temperature Range | Up to 80℃ between wells |
| Fluorescence Detection | 488 nm laser excitation
520 nm emission from sample For use with SyBR green, EvaGreen, FAM dye |
| Automation Communication | API and .csv file transfer |
| Automation Compatibility | Standard liquid handling robot-compatible |
| Software | Windows compatible SW |
| Intended Use | For research use only |
Downloads
Gain control of your PCR at the individual well level with AutoNorm™
iconPCR brochure
Contact us for application specific workflow & app notes
Unlocking Superior Microbial Profiling with iconPCR™
Support hundreds of diverse projects each month with 50% less hands-on time
Sequencing (DNA or RNA-Seq) from FFPE / Degraded Samples
Streamline FFPE workflows, reduce failures and wasted sequencing, and rescue samples often lost with conventional PCR for more reliable results and consistent yields.
Read Balancing
iconPCR ensures every sequencing pool is perfectly balanced-no more wasted reads, reruns, or surprises.
Index Performance Evaluation
How well do you know your indexes? Utilize iconPCR for index PCR to reveal differences in performance and help alleviate issues.
Do you want an in-person demo of this product?
Please do not hesitate to reach out to me if you have technical questions or need price estimates for any of our products and services. We are just a phone call away!