Tapestri® Single-Cell DNA & Multiomics Platform
Connect genotype to phenotype – one cell at a time
Bulk sequencing provides an average across a mixed population of cells. The Mission Bio Tapestri Platform reveals the underlying clonal architecture by identifying which genomic alterations, and when required, surface proteins or targeted gene-expression patterns occur together in the same individual cells.
- Targeted single-cell DNA, DNA + protein and DNA + RNA workflows
- Detect SNVs, indels, CNVs, LOH and translocations
- Resolve rare subclones across thousands of individual cells
- Choose predesigned, published or fully customized panels
- Move from single-cell suspension to sequencing-ready libraries and analysis
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Product overview
Go beyond the average
A bulk sequencing result can show that several mutations are present in a sample, but it cannot reliably establish whether those mutations occur together in the same cells or in separate cell populations.
Tapestri analyzes thousands of cells individually. This makes it possible to identify:
- Rare cell populations and subclones
- Mutational co-occurrence
- Variant zygosity
- Clonal architecture and clonal evolution
- Genomic drivers associated with response, resistance or relapse
- Relationships between genotype, protein expression and targeted gene expression
The platform is particularly valuable when cellular heterogeneity is central to the research question, for example in oncology, hematology, genome editing and cell and gene therapy.
Mission Bio lists SNVs, indels, CNVs, loss of heterozygosity and translocations among the genomic events that can be characterized with Tapestri panels.
One platform, multiple levels of information
Single-cell DNA
Identify genomic variants in individual cells and determine which variants coexist within the same clone.
DNA + protein
Measure targeted genomic alterations and cell-surface protein expression from the same cells. This connects genotype with immunophenotype and helps distinguish genetically similar but phenotypically different cell populations.
Targeted DNA + RNA
Measure targeted DNA variants and selected gene-expression targets in the same cells. This provides a direct link between genomic alterations and their functional transcriptional effects without the sequencing burden of whole-transcriptome analysis.
Copy-number analysis
Characterize focal, gene-level, chromosome-arm or genome-wide copy-number alterations together with targeted sequence variants.
The DNA + RNA workflow is targeted rather than whole-transcriptome sequencing. Mission Bio introduced the commercial targeted DNA + RNA workflow and corresponding user guides during 2025–2026.
How the Tapestri workflow works
1. Select your targets
Choose a wet-lab-tested catalog panel, a published panel, a customizable virtual panel or create a fully custom panel using Tapestri Designer.
2. Prepare the sample
Prepare a debris-free single-cell or single-nuclei suspension. For DNA + protein experiments, cells are stained with compatible oligo-conjugated antibodies before loading.
3. Encapsulate and barcode cells
The Tapestri Instrument uses a two-step microfluidic workflow. Individual cells are encapsulated, lysed and paired with cell-specific molecular barcodes.
4. Perform targeted amplification
Selected genomic – and depending on the assay – protein or RNA, targets are amplified while retaining the identity of the cell from which each signal originated.
5. Sequence the libraries
The resulting sequencing-ready libraries are processed using a supported NGS workflow.
6. Analyze and visualize
Tapestri Pipeline converts FASTQ files into single-cell outputs. Mosaic and application-specific pipelines can then be used to visualize clones, variants, copy-number changes and multiomic relationships.
The platform sits upstream of the NGS instrument and includes instrument, reagents, consumables and analysis tools. Tapestri Pipeline is available through cloud-hosted or on-premise configurations, while Mosaic supports customizable downstream exploration.
Key applications
Hematologic malignancy research
Resolve clonal composition and evolution in AML, MDS, MPN, multiple myeloma, CLL and other hematological diseases. Identify mutation combinations, rare subclones and changes between longitudinal samples.
Measurable residual disease research
Investigate residual cell populations by combining mutational profiles with phenotypic information at single-cell resolution.
Solid-tumor profiling
Analyze dissociated cells or nuclei from fresh or frozen tissue to investigate SNVs, indels, copy-number changes, zygosity and mutational co-occurrence.
Genome editing
Measure intended edits, unedited cells, multiple editing outcomes, translocations and other low-frequency events in individual cells.
Cell and gene therapy
Assess editing or gene-transfer heterogeneity across a cell population and connect genomic outcomes with protein or gene-expression profiles.
Biomarker and drug-development research
Characterize mechanisms of action, resistance and response by following biologically relevant clones and their phenotypes.
Mission Bio currently positions Tapestri for hematology, solid tumors, genome editing, precision medicine and cell and gene therapy research.
Specifications
| Parameter | Specification |
|---|---|
| Technology | Two-step droplet-based microfluidic single-cell encapsulation and barcoding |
| Primary assay modes | Targeted single-cell DNA; DNA + surface protein; targeted DNA + RNA |
| Genomic measurements | SNVs, indels, focal and genome-wide CNVs, LOH and translocations |
| Sample format | Debris-free single-cell or single-nuclei suspension |
| Common sample types | Cell lines, PBMCs, bone-marrow-derived cells, dissociated solid-tumor cells and nuclei from fresh or frozen tissue |
| Recommended cell viability | More than 80% for viable-cell workflows |
| Recommended loading concentration | Approximately 2,800–3,200 cells or nuclei/µL |
| Recommended material before cartridge loading | Approximately 30,000–100,000 cells; higher starting amounts may be required because of washing and processing losses |
| Maximum cell size | Less than 30 µm |
| Panel options | Catalog, published, virtual-design and fully custom panels |
| Custom DNA-panel capacity | From focused panels up to approximately 1,000 amplicons |
| Reference genomes | Human, mouse or other supported custom reference genomes |
| Sample multiplexing | Up to three samples per experiment using genotype-based multiplexing or antibody hashing |
| Sequencing | Sequencing-ready libraries for supported Illumina workflows; configuration depends on assay and panel |
| Secondary analysis | Tapestri Pipeline |
| Advanced exploration | Mosaic and application-specific pipelines |
| Pipeline deployment | Cloud-hosted or on-premise options |
| Instrument dimensions, H × W × D | 31.75 × 29.85 × 31.10 cm |
| Instrument weight | Approximately 6.35 kg |
| Power | 100–240 V AC, 50–60 Hz |
| Typical operating power | Approximately 60 W |
| Operating temperature | 15–30°C |
| Relative humidity | 5–85%, non-condensing |
| Intended use | For Research Use Only. Not for use in diagnostic procedures |
FAQ
What is the Mission Bio Tapestri Platform?
Tapestri is a targeted single-cell DNA and multiomics platform. It uses microfluidics and cell-specific molecular barcodes to identify genomic alterations—and optionally surface proteins or targeted RNA expression—in thousands of individual cells.
How is Tapestri different from bulk NGS?
Bulk NGS averages signals across all cells in a sample. It can show that two mutations are present, but it may not reveal whether they occur in the same clone.
Tapestri retains the identity of each cell. This makes it possible to determine mutation co-occurrence, zygosity, clonal structure and the presence of rare subpopulations.
Is Tapestri a single-cell RNA-sequencing platform?
Tapestri supports targeted single-cell DNA + RNA analysis. It is not primarily a whole-transcriptome discovery platform.
The targeted approach is useful when you already know which genomic and expression targets are relevant and want to connect variants with gene expression in the same cells.
What types of variants can Tapestri detect?
Depending on the panel and workflow, Tapestri can characterize:
- Single-nucleotide variants
- Insertions and deletions
- Copy-number variants
- Loss of heterozygosity
- Translocations
- On-target and selected off-target genome-editing outcomes
Can Tapestri measure DNA and protein in the same cell?
Yes. Compatible oligo-conjugated antibodies can be combined with the DNA workflow to measure genomic alterations and cell-surface proteins in the same individual cells.
This allows researchers to connect genotype with immunophenotype rather than analyzing DNA and protein in separate cell populations.
Can I create a custom panel?
Yes. Tapestri Designer can be used to explore catalog content or build custom targeted panels.
Panels can be designed for human, mouse or other supported reference genomes and can include SNPs, genomic regions, editing targets and copy-number targets. Mission Bio’s current software supports designs ranging from focused panels to approximately 1,000 amplicons.
What samples can be used?
Common inputs include:
- Cultured cell lines
- PBMCs
- Bone-marrow-derived cells
- Cryopreserved cells
- Dissociated fresh tissue
- Single nuclei isolated from fresh, frozen or OCT-embedded tissue
- Cells isolated from solid tumors
Compatibility varies between DNA-only, DNA + protein and DNA + RNA workflows, so sample suitability should be reviewed before starting the project.
Can whole blood be loaded directly?
No. Whole blood is not a direct input. The relevant cells must first be isolated and prepared as a debris-free single-cell suspension.
Mission Bio recommends approximately 3,000 cells/µL and more than 80% viability for viable-cell workflows.
Are FFPE samples supported?
FFPE samples are not currently supported as direct input for the standard Tapestri workflow. Fresh, cryopreserved, fresh-frozen or OCT-embedded material may be suitable depending on the assay and preparation method.
How many cells are required?
Mission Bio recommends having approximately 30,000–100,000 cells immediately before cartridge loading.
Because cells are lost during washing, counting and staining, the starting sample should normally contain more cells. DNA + protein experiments may require up to approximately one to two million cells at the beginning of the preparation workflow.
Can multiple samples be processed together?
Yes. Up to three samples can be combined in one experiment using genotype-based multiplexing or antibody hashing. Tapestri Pipeline then automatically separates the samples during analysis.
Which sequencing system is required?
Tapestri produces sequencing-ready libraries for supported Illumina sequencing workflows. The appropriate instrument, flow cell, read configuration and sequencing depth depend on the panel, assay and expected number of cells.
Configure the right Tapestri solution for your project
Tapestri configurations depend on your application, sample type, assay modality, panel content, sample volume, sequencing system and analysis requirements.
To help us prepare the correct recommendation and quotation, please include:
- Research application
- Sample type and preparation method
- Number of samples and time points
- Estimated number of available cells or nuclei
- Required assay: DNA, DNA + protein or DNA + RNA
- Genes, variants or genomic regions of interest
- Requirement for a catalog or custom panel
- Available sequencing instrument
- Preferred cloud or on-premise analysis
- Expected project start date
Techtum can assist with instrument configuration, panels, reagent kits, consumables, installation, training, sequencing planning and analysis requirements.
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